Having been on the other side of the fence (i.e. an interviewer), some of the basic things I take note (other than some of Dani & Scott's great pointers) are :
a) Punctuality :
- If you're late, it shows how much of "interest" you have in the job.
- Suppose you've very valid reasons, please make it a point to apologize and do a brief explanation.
b) Eye Contact & Body Language :
- Do make it a point to have eye contact with the interviewer. I usually associate drifty eyes as a sign of low attention span and lack of interest.
- When questions are being posed on you and you find it ambiguous, please ask the interviewer to repeat the question nicely and do not sound "irritated". Some questions are asked to test your situational awareness and if you sound irritated or so, it goes to imply a low level of EQ and a lack of patience.
c) Ask the Right Questions
- Feel free to clarify the scope of the job, remumeration matters etc during the interview but do avoid asking questions that
(i) has already been asked. Gosh, it gives the interviewer the impression that you were not paying attention to what was being mentioned previously
(ii) are irrelevant such as how many candidates they are interviewing etc.
Just my 2cents

In any case, derek, all the best and do let us know if you get the job!